Join me @ IBOtoolbox for free.
Chuck Reynolds
Member Since: 12/6/2012
performance / stats
Country: United States
Likes Received: 1693
Featured Member: 0 times
Associates: 2326
Wall Posts: 2026
Comments Made: 1497
Press Releases: 1998
Videos: 1
Phone: 559-369-7791
Skype:     chuck212
profile visitor stats
TODAY: 122
TOTAL: 392496
are we ibo associates?
recent videos
active associates
Malcolm Patten    
Last logged on: 7/23/2019

Nigel Welford    
Last logged on: 7/23/2019

John Kespert    
Last logged on: 7/23/2019

Chris Shouse    
Last logged on: 7/23/2019

IBOtoolbox Admin     
Last logged on: 7/23/2019

Sumaya Kabbara El Ayoubi    
Last logged on: 7/23/2019

Danny Dreamss     
Last logged on: 7/23/2019

Leo Zavala    
Last logged on: 7/23/2019

Anne Pinney    
Last logged on: 7/23/2019

Chow Keng Sun    
Last logged on: 7/23/2019

Ostell Hodges Jr    
Last logged on: 7/23/2019

Roman Spoljar  
Last logged on: 7/23/2019

Katarina Hofbaur  
Last logged on: 7/23/2019

James Doan       
Last logged on: 7/23/2019

Last logged on: 7/23/2019

other ibo platforms

Chuck Reynolds   My Press Releases

What does Resveratrol induce.? P-9

Published on 6/17/2019
For additional information  Click Here

Resveratrol induces autophagy by directly inhibiting mTOR through ATP competition P-9

Resveratrol inhibits mTOR through ATP competition

To verify the simulation results, we performed an in vitro kinase assay with various concentrations of ATP. PP242, a kinase inhibitor of mTOR, was used as a positive control for mTOR inhibition. We found that resveratrol-induced mTOR inhibition was restored by the addition of ATP. To clarify whether resveratrol inhibits mTOR through direct interaction, we generated a resveratrol-resistant mTOR mutant. Based on the resolved mTOR-ATP structure and our computational simulation. Asp 2195 was deemed to be a resveratrol-binding residue that does not affect the mTOR-ATP interaction.

Thereafter, we generated an mTOR mutant in which Asp 2195 was substituted with alanine (hereafter, referred to as D2195A) and performed an in vitro kinase assay. As expected, resveratrol inhibited wild-type (WT) mTOR but not D2195A mTOR. The basal activity level of the D2195A mutant was lower than that of WT mTOR, possibly due to the fact that the mutated residue resides in the kinase domain of the protein. We observed that PP242 also inhibited DA mTOR, albeit the inhibitory potency was less than WT mTOR. Although Asp 2195 was suggested as a residue for the interaction between mTOR and PP242, other residues also participate the interaction between them and not all residues are shared between PP242 and resveratrol for mTOR binding.

We speculate that other residues but not D2105 may be essential for the PP242 binding to mTOR and that is why D2195A mTOR was not completely resistant to PP242. To examine whether the resveratrol resistant mTOR blocks the resveratrol-induced autophagy, we measured the autophagy levels upon resveratrol treatment in the WT or D2195A mTOR along with Raptor transfected cells. We found that resveratrol induced autophagy in the WT mTOR transfected cells, but not in the D2109A mTOR transfected cells. Taken together, the results from the experiments using resveratrol resistant mTOR suggest that resveratrol induces autophagy by directly inhibits mTOR through ATP competition.

Article Produced By
Department of Life Sciences, Pohang University of Science and Technology, Pohang 790-784, Republic of Korea
School of Interdisciplinary Bioscience and Bioengineering, Pohang University of Science and Technology, Pohang 790-784, Republic of Korea
School of Life Sciences, Ulsan National Institute of Science and Technology, Ulsan 689-798, Republic of Korea
Department of Bioscience and Biotechnology, Sejong University, 98 Kunja-Dong, Kwangjin-Ku, Seoul 143-747, Republic of Korea

(All these Articles can be read with this URL: )

Posted by
Chuck Reynolds

The reasoning for this article is to supply you information about this wonderful product and also for me to find at least two or more people for this simply Amazing Opportunity.. You just need to follow the 4 simple steps..  (Join,Train,sigup 2 or more people and then you duplicate. It's that simple..). This business can make you money in the thousands or just be a customer for a very satisfying experience.

View this video as it helps you to understand a very simple Business Plan. Check it out, then
View these videos (4 of them, very short (total less then 5 min)) that explains how simple
this business works and if so inclined, click on my name above, call me or just sign up
either as a Customer or Distributor.

Thank You

Member Note: To comment on this PR, simply click reply on the owners main post below.
-  Copyright 2016 IBOsocial  -            Part of the IBOtoolbox family of sites.